Triacontanol Formulation (0.1% w/w) - Agro Chemical Formulations

Triacontanol is a naturally occurring compound and is considered non-toxic to plants, animals, and humans. It influences the levels of various plant hormones, including auxins, gibberellins, and cytokinins, which play crucial roles in growth and development. Although, application of Triacontanol is a well-known agricultural practice, recent studies have demonstrated that its use can prove to be beneficial for enhanced micropropagation in tissue culture: 

Specific advantages of Triacontanol Application of in Micropropagation of Plant Tissue Culture

  1. Enhancement of Shoot and Root Growth:

Triacontanol has been shown to promote shoot proliferation and root initiation in micropropagated tissues. It increases the number and length of shoots and roots, thereby improving overall plantlet development.

  1. Increase in Biomass Production:

Its application often results in increased biomass accumulation in tissue cultures, which is beneficial for scaling up propagation protocols.

  1. Improvement of Callus Induction and Growth:

Triacontanol can enhance callus induction rates and the vigor of callus tissues, leading to more efficient regeneration processes.

  1. Reduction in Culture Duration:

By stimulating metabolic activities, triacontanol may shorten the time needed for successful micropropagation, making the process more efficient.

  1. Enhancement of Nutrient Utilization:

It improves nutrient uptake and assimilation, supporting better growth conditions in vitro.

  1. Stress Tolerance and Morphogenic Responses:

Triacontanol has been reported to increase stress tolerance and promote morphogenic responses such as organogenesis and somatic embryogenesis in some plant species.

  1. Synergistic Effects with Other Growth Regulators:

Often used in combination with auxins and cytokinins to optimize tissue culture media, leading to improved regeneration outcomes.

Specific Examples in literature citing Triacontanol Applications:

 

Crop

Concentration

Effect

1.

Banana (Musa spp)

45ppb – 2ppm

a.    Shoot Proliferation: 

Enhancement of shoot multiplication in banana cultivars like Musa spp. by stimulating cytokinin-like activity.  Resulting in  Increased shoot numbers (30–50%) and improved shoot vigor compared to cytokinin-only media.

b.    Stress Mitigation: 

TRIA reduces oxidative stress in banana cultures exposed to heavy metals (e.g., cadmium) by boosting antioxidant enzymes like SOD and CAT

2.

Potato (Solanum tuberosum)

40 ppm – 1 ppm

a.    Microtuber Induction:

Promotes microtubers by enhancing sucrose utilization and starch biosynthesis.  Resulting in higher tuber counts (up to 50% increase) and larger tuber size.

b.    Synergy with Jasmonic Acid: 

Combined with Jasmonic acid, TRIA accelerates tuberization and improves carbohydrate storage

3.

Stevia (Stevia rebaudiana)

400 ppb – 4.5 ppm

Boosts shoot biomass and steviol glycoside production (e.g., rebaudioside-A) by upregulating diterpenoid biosynthesis pathways

4.

Ashwagandha (Withania somnifera)

 

Enhances root biomass and withanolide synthesis (key medicinal compounds) in vitro by modulating stress-responsive genes

Note: Its role as a supplement in culture media is often at low concentrations (generally around 400 ppb – 4 ppm), where it acts as a biostimulant rather than a primary growth regulator. WARNING: Excess TRIA (> 5ppm) may inhibit growth or cause callus hyperhydricity. 

Disclaimer

This information is provided for general reference only and is based on publicly available sources. Although due care has been taken to ascertain the reliability of sources, Pure Check India makes no guarantees regarding its accuracy or completeness and advocates independent verification of critical details by the user to ascertain suitability for any specific application.

PURABOOST- Triacontanol

PURABOOST™ is a formulation containing Triacontanol (0.1% w/w) as an emulsifying concentrate in water. Triacontanol is a naturally occurring compound derived from vegetable waxes. It is considered non-toxic to plants, animals, and humans. Used at recommended concentrations, Triacontanol influences the levels of various plant hormones, including auxins, gibberellins, and cytokinins, which play crucial roles in growth and development. Its spraying is generally recommended during the following key growth stages of flowering plants to maximize benefits: 

  1. Early Vegetative Stage: to promote overall plant growth, including root and shoot development, leading to a stronger foundation for flowering.
  2. Pre-Flowering/Flowering Initiation Stage: to enhance the number of flower buds, promote uniform flowering, and improve the potential for fruit set
  3. Fruit Setting Stage: to help reduce flower and fruit drop, leading to a higher yield
  4. Fruit Development/Grain Filling Stage: to improve the size, quality, and overall yield of the produce.

 Benefits:

  1. Enhanced Photosynthesis: Triacontanol increases chlorophyll content thereby improves the efficiency of photosynthesis, leading to more energy production for the plant.
  2. Stimulated Growth and Development: It promotes cell division and elongation, resulting in faster germination, robust root development, and vigorous shoot growth.
  3. Improved Flowering and Fruit Set: Triacontanol increases the number of flower-buds, promotes uniform flowering, and reduces flower and fruit drop, leading to higher yields.
  4. Increased Nutrient Uptake: Triacontanol enhances the permeability of plant cell membranes, facilitating the absorption and utilization of essential minerals and nutrients from the soil.
  5. Enhanced Stress Tolerance: Triacontanol helps plants withstand environmental stresses such as drought, heat, and salinity.

Recommended Dilution and Dosage:

2.0 ml/L water for foliar spray

500 ml/ acre for drip irrigation

500ml in 500L water for big trees like mango

Formulation Ingredient List:

  1. Triacontanol – 0.1% w/w
  2. Ethoxylated esters of Fatty acids – 0.5% w/w
  3. Esters of Benzoic acid – 0.1% w/w
  4. Demineralized water – QS